Erythrocytes selectively sensitized with c3 complement...

Details Erythrocytes selectively sensitized with c3 complement... Erythrocytes selectively sensitized with c3 complement...

G - Physics

01

N

167/45

G01N 33/48 (2006.01) G01N 33/555 (2006.01) G01N 33/68 (2006.01) G01N 33/96 (2006.01)

Patent

CA 1114743

Abstract A set of four cell suspensions containing erythrocytes monospeci- fically sensitized with IgG, IgM, C3, and C4. The suspensions provide control cells to determine the purity and strength of the antibodies to these immunoglobins or complement components contained in antisera used for clinical testing. Strict control of the pH, temperature, and EDTA concentration of a solution to which blood is added allows for the elective production of C3- or C4- sensitized cells. Selecting a serum with IgG or IgM antibodies and inactivating undesired proteins permits the production IgG- or IgM-sensitized cells. The resultant cells have agglutination reaction strengths of about 2-4+. Erythro- cytes from humans or other animals may undergo the monospecific sensi- tization. Other human proteins, such as IgA or CS, and plasma proteins from other animals may similarly be attached monospecifically to red blood cells.

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