Erythrocytic cells and method for preserving cells

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 5/00 (2006.01) A01N 1/02 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2454910

A dehydrated composition is provided that includes freeze-dried erythrocytic cells. Alcohol (e.g., sterol or cholesterol) is at least partially removed from erythrocytic cells including erythrocytic membranes. After removal of at lest part of the alcohol, the erythrocytic cells have a low phase transition temperature range, an intermediate phase transition temperature range, and a high phase transition temperature range. The erythrocytic cells may be loaded with an oligosaccharide (e.g., threhalose) which preserves biological properties during freeze-drying and rehydration. A process for increasing cooperativity of a phase transition of an erythrocytic cell. A process for preserving and/or increasing the survival of dehydrated erythrocytic cells, including storing dehydrated erythrocytic cells having a residual water content equal to or less than about 0.30 gram of water per gram of dry weight erythrocytic cells.

L'invention concerne une composition déshydratée comprenant des cellules érythrocytaires lyophilisées. De l'alcool (par exemple du stérol ou du cholestérol) est au moins partiellement éliminé des cellules érythrocytaires, y compris les membranes érythrocytaires. Suite à l'élimination d'au moins une partie de l'alcool, les cellules érythrocytaires présentent une plage de température de transition de phase basse, une plage de température de transition de phase intermédiaire, et une plage de température de transition de phase élevée. Les cellules érythrocytaires peuvent être chargées avec un oligosaccharide (par exemple, le thréhalose) préservant les propriétés biologiques au cours de la lyophilisation et de la réhydratation. L'invention concerne également un procédé augmentant l'ajustement d'une transition de phase d'une cellule érythrocytaire. L'invention concerne enfin un procédé permettant de préserver et/ou d'augmenter la survie de cellules érythrocytaires déshydratées, consistant à stocker des cellules érythrocytaires déshydratées comprenant un contenu en eau résiduelle égal ou inférieur à environ 0,30 grammes d'eau par gramme de cellules érythrocytaires en poids sec.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Erythrocytic cells and method for preserving cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Erythrocytic cells and method for preserving cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Erythrocytic cells and method for preserving cells will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1514475

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.