C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/00 (2006.01) A61K 39/108 (2006.01) A61K 39/116 (2006.01) A61K 39/385 (2006.01) A61K 47/48 (2006.01) C07K 2/00 (2006.01)
Patent
CA 2115564
The present invention relates to a method of producing an E. coli vaccine and to the vaccine produced thereby. The method involves purifying lipopolysaccharide from E. coli expressing complete O- polysaccharide sidechains; isolating the O-polysaccha- ride region of the lipopolysaccharide molecule by hydrolysis in dilute acetic acid and purifying it essentially free of lipid A; and covalently coupling lipid A-free O-polysaccharide via at least one hydroxyl or carboxyl group of the polysaccharide to a carrier protein. Polyvalent vaccines are prepared by combining two or more monovalent vaccines for different serotypes prepared ac- cording to the present invention. The present invention also relates to conjugates used in the vaccines. The conjugates of the pres- ent invention are the O-polysaccharide region of an E. coli lipopolysaccharide molecule covalently coupled to a carrier protein.
Cryz Stanley J. Jr.
Furer Emil P.
Cryz Stanley J. Jr.
Gowling Lafleur Henderson Llp
Swiss Serum And Vaccine Institute Berne
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