C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2462076
A plant gene, Esr2, has been found which when overexpressed in plant cells results in cells which have cytokinin-independent cell growth. This feature allows the encoded protein ESR2 to be used as a selectable marker of transformed cells by growing the transformed cells on cytokinin-free media. It has also been found that overexpression of ESR2 in cells grown in the presence of cytokinins results in a higher regeneration of plants. This feature allows the gene to be used to obtain greater regeneration of plant cells.
L'invention concerne un gène de plante, Esr2, qui, lorsqu'il est surexprimé dans des cellules végétales, produit des cellules présentant une croissance cellulaire indépendante des cytokinines. Cette caractéristique permet d'utiliser la protéine ESR2 codée comme marqueur de sélection spécifique de cellules transformées, par la culture de ces cellules transformées sur des supports exempts de cytokinines. La surexpression de l'ESR2 dans des cellules, en présence de cytokinines, produit également une meilleure régénération des plantes. Cette caractéristique permet d'utiliser le gène selon l'invention pour obtenir une meilleure régénération des cellules végétales.
Chua Nam-Hai
Ikeda Yoshihisa
Osler Hoskin & Harcourt Llp
The Rockefeller University
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