Esterase genes, esterase, recombinant plasmids and...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/18 (2006.01) C12P 7/40 (2006.01) C12P 11/00 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2068614

The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, sand esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); Image (wherein R1 is alkyl, aralkyl or aryl, R2 and R3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.

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