Estimation of activity or inhibition of processes involved...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2533572

A method for determining the activity of a substance capable of altering the structure of a nucleic acid molecule from a first to a second state which is based upon the use of labelled nucleic acid molecules and/or complementary oligonucleotides. The label is a chemiluminescent molecule and a corresponding quencher molecule whose optical properties are different depending upon whether the nucleic acid molecule exist in said first or second state.

L'invention concerne un procédé permettant de déterminer l'activité d'une substance capable de modifier la structure d'une molécule d'acide nucléique pour la faire passer d'un premier à un second état. Ce procédé repose sur l'utilisation de molécules d'acide nucléique et/ou d'oligonucléotides complémentaires marqués. Le marqueur est composé d'une molécule chimioluminescente et d'une molécule d'extinction correspondante, dont les propriétés optiques sont différentes selon que la molécule d'acide nucléique se trouve dans le premier ou dans le second état.

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