Estrogen dependent transactivation system for gender sorting...

Details Estrogen dependent transactivation system for gender sorting... Estrogen dependent transactivation system for gender sorting...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 14/72 (2006.01) C12N 1/19 (2006.01) C12Q 1/02 (2006.01) C12Q 1/34 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2445162

The disclosure relates to methods of detecting target molecules, such as hormones, using a hormone-dependent transactivation system. The disclosure also includes a sex hormone-dependent transactivation system and kit for gender sorting in avian species, which provides a quick, inexpensive, automated method to sex segregate avian eggs before hatching. Genetically engineered host cells containing the sex hormone-dependent transactivation system are contacted with a biological sample, and the engineered cells respond to the level of sex hormone in the biological sample by activating a reporter gene, which is indicative of the presence of the sex hormone. The disclosure further provides methods of gender sorting in avian species using the sex hormone-dependent transactivation system, which enables pre-hatch sorting of female embryos from male embryos.

L'invention concerne un processus de transactivation à base de levures, permettant une sélection sexuelle.

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