C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/06 (2006.01) C12M 1/02 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/24 (2006.01) C12N 9/88 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2106377
Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising gen- es encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts de- scribed above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing bio- mass to ethanol.
Beall David S.
Ben-Assat Arie
Burchhardt Gerhard F. H.
Fowler David E.
Guimjaraes Walter V.
Bioenergy International L.c.
Smart & Biggar
University Of Florida
University Of Florida Research Foundation Inc.
Verenium Corporation
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