C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C07K 1/00 (2006.01) C12N 1/18 (2006.01) C12P 7/08 (2006.01)
Patent
CA 2567109
The present invention relates to an ethanol producing microbial strain, such as Saccharomyces cerevisiae strain, being able to grow and produce ethanol from lignocellulosic hydrolysates comprising growth inhibiting compounds of the group furfural and 5- hydroxy-methyl furfural, in a batch, fed-batch or continuous fermentation, said microbial strain being tolerant to such inhibiting compounds, which strain is upregulated and/or over expressed with regard to one or more of the following genes: LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1.
L'invention concerne une souche microbienne produisant de l'éthanol, telle que la souche Saccharomyces cerevisiae, qui peut croître et produire de l'éthanol à partir d'hydrolysats lignocellulosiques comprenant des composés inhibiteurs de croissance qui appartiennent au groupe furfural et 5- hydroxy-méthyl furfural, en fermentation discontinue, en fermentation à écoulement discontinu ou en fermentation continue. Ladite souche microbienne tolère de tels composés inhibiteurs et est régulée à la hausse et/ou surexprimée par rapport à un ou plusieurs des gènes suivants: LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1.
Gorwa-Grauslund Marie-Francoise
Hahn-Haegerdal Baerbel
Liden Gunnar
Modig Carl Tobias
Moreira de Almeida Joao Ricardo
Forskarpatent I. Syd Ab
Mccarthy Tetrault Llp
Scandinavian Technology Group Ab
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