Eukaryotic cell-based gene interaction cloning

Details Eukaryotic cell-based gene interaction cloning Eukaryotic cell-based gene interaction cloning

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/71 (2006.01) C07K 14/715 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2337086

The present invention relates to a method for screening compounds for their ability to bind a receptor and/or the screening of compounds that antagonise the binding of a ligand to a receptor. It is the aim of the present invention to provide an easy and powerful screening method in eukaryotic cells, such as insect cells, plant cells or mammalian cells, with the exclusion of yeast cells, for ligands of orphan receptors, preferentially of the multimerizing receptor type, for unknown ligands of known receptors, preferentially multimerizing receptors and for the genes encoding these ligands.

La présente invention concerne une méthode de recherche systématique, chez certains composés, d'une aptitude à se lier à un récepteur, et/ou de composés qui s'opposent à la liaison d'un ligant avec un récepteur. Le but de cette invention est de fournir un outil simple et puissant de recherche systématique, chez des cellules eukaryiotes telles que cellules d'insectes, de végétaux ou de mammifères (mais pas de levures), de ligands pour récepteurs orphelins, de préférence du type multimérisant, de ligands inconnus de récepteurs connus, de préférence du type multimérisant, et pour des gènes codant pour ces ligands.

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