C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) C12N 5/0775 (2010.01) A01N 1/02 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) C12N 1/04 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2454684
A dehydrated composition is provided that includes freeze-dried eukaryotic cells. The eukaryotic cells are loaded with an oligosaccharide (e.g., trehalose) which preserves biological properties during freeze-drying and rehydration. The oligosaccharide loading is conducted at a temperature of from greater than about 25~C to less than about 50~C, more preferably at about 35~C, with the loading solution having the oligosaccharide in an amount from about 10mM to about 100mM. These freeze-dried eukaryotic cells are rehydratable. A process for preserving and/or increasing the survival of dehydrated eukaryotic cells, including storing dehydrated eukaryotic cells having a residual water content greater than about 0.15 gram of water per gram of dry weight eukaryotic cells.
L'invention concerne une composition déshydratée contenant des cellules eucaryotes lyophilisées. Ces cellules eucaryotes sont chargées au moyen d'un oligosaccharide (du tréhalose, par exemple) qui conserve les propriétés biologiques pendant la lyophilisation et la réhydratation. La charge d'oligosaccharide est effectuée à une température supérieure à environ 25 ·C et inférieure à environ 50 ·C, de préférence à environ 35 ·C, la solution de charge contenant l'oligosaccharide étant présente dans une quantité comprise entre environ 10 mM et environ 100 mM. Les cellules eucaryotes lyophilisées selon l'invention peuvent être réhydratées. L'invention concerne également un procédé permettant de conserver et/ou d'augmenter la survie des cellules eucaryotes déshydratées, notamment de stocker les cellules eucaryotes déshydratées présentant une teneur en eau résiduelle supérieure à environ 0,15 grammes d'eau par gramme de cellules eucaryotes en poids sec.
Crowe John H.
Htoo Thurein
Jamil Kamran
Olivier Ann E.
Tablin Fern
Smart & Biggar
The Regents Of The University Of California
LandOfFree
Eukaryotic cells and method for preserving cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Eukaryotic cells and method for preserving cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Eukaryotic cells and method for preserving cells will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1742711