Evolution of whole cells and organisms by recursive sequence...

Details Evolution of whole cells and organisms by recursive sequence... Evolution of whole cells and organisms by recursive sequence...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2276064

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

La présente invention se rapporte à des procédés mettant en oeuvre des cycles itératifs de recombinaison et de sélection/criblage ayant pour but de faire évoluer des cellules entières et des organismes de manière à ce qu'ils acquièrent des propriétés désirées. De telles propriétés incluent la recombinogénicité, le nombre de copies du génome et la capacité d'expression et/ou de sécrétion de protéines et de métabolites secondaires.

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