Ex vivo and in vivo expression of the thrombomodulin gene...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 31/70 (2006.01) A61K 35/76 (2006.01) A61K 38/36 (2006.01) C12N 15/861 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2508514

The present invention relates to methods and compositions for treatment of cardiovascular and peripheral vascular diseases using ex vivo and in vivo gene delivery technologies. One aspect of the present invention relates to a method for treating a vascular disease by introducing a DNA sequence encoding a TM protein or its variant into a segment of a blood vessel ex vitro using a gutless adenovirus vector. Another aspect of the present invention is to provide a method to deliver a gutless adenovirus vector carrying a DNA sequence encoding a TM protein or its variant using a stent.

La présente invention concerne des procédés et des compositions pour traiter des maladies cardiovasculaires et des maladies vasculaires périphériques au moyen de technologies de transport de gène ex vivo et in vivo. Un aspect de cette invention concerne un procédé pour traiter une maladie vasculaire en introduisant une séquence d'ADN codant une protéine TM ou sa variante dans un segment d'un vaisseau sanguin ex vitro, au moyen d'un vecteur d'adénovirus n'exprimant plus de gènes viraux. Un autre aspect de cette invention concerne un procédé pour transporter un vecteur d'adénovirus n'exprimant plus de gènes viraux, qui porte une séquence d'ADN codant une protéine TM ou sa variante, au moyen d'une endoprothèse.

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