Expansion of stem and progenitor cells by beta-catenin

Details Expansion of stem and progenitor cells by beta-catenin Expansion of stem and progenitor cells by beta-catenin

A - Human Necessities

01

N

A01N 1/02 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 7/00 (2006.01) C12N 15/63 (2006.01) C12N 15/86 (2006.01) C12P 21/04 (2006.01) C12P 21/06 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2396576

Mammalian progenitor or stem cells are expanded in vitro by increasing the levels of .beta.-catenin in the cell. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. The intracellular levels of .beta.- catenin may be manipulated by providing exogenous .beta.-catenin protein to the cell, or by introduction into the cell of a genetic construct encoding .beta.-catenin. The .beta.-catenin may be a wild-type or stabilized mutant form of the protein. Preferably the long term cell culture medium substantially lacks stromal cells and cytokines.

Selon l'invention, des cellules souches ou précurseurs mammaliennes sont amplifiées in vitro par augmentation des niveaux de la .beta.-caténine dans la cellule. Les cellules amplifiées conservent sensiblement leur phénotype originel, notamment leur aptitude à donner naissance à plusieurs types de cellules différenciées. Les niveaux intracellulaires de la .beta.-caténine peuvent être manipulés par apport d'une protéine exogène de la .beta.-caténine à la cellule, ou par introduction dans la cellule d'une construction génétique codant la .beta.-caténine. La .beta.-caténine peut être de type sauvage ou une forme mutante stabilisée de la protéine. De préférence, le milieu de culture cellulaire à long terme manque sensiblement de cellules du stroma et de cytokines.

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