Exponential amplification of nucleic acids using nicking agents

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2492423

The present invention provides methods and compositions for exponential amplification of nucleic acid molecules using nicking agents. In certain aspects, the amplification may be performed isothermally. This invention is useful in many areas such as disease diagnosis.

La présente invention concerne des méthodes et des compositions destinées à une amplification exponentielle de molécules d'acides nucléiques au moyen d'agents de croisement spécifique favorable. Selon certains aspects, ladite amplification peut être réalisée de manière isotherme et elle peut être utilisée dans plusieurs domaines, tels que le diagnostic de maladies.

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