Expressing gp140 fragment of primary hiv-1 isolate

C - Chemistry – Metallurgy – 12 – N

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C12N 15/49 (2006.01) A61K 48/00 (2006.01) C07K 14/16 (2006.01)

Patent

CA 2363704

A vector for eliciting an immune response to a host comprising a gene encoding the gp140 protein of the primary isolate of HIV-1, BX08, under the control of a promotor for expression of the protein in the host, specifically plasmids pCMV.gp140.BX08, pMP83, pMP84 and pMP88. Murine and human MHC class 1- restricted binding motifs contained in BX08 are identified.

L'invention porte sur un vecteur utilisé pour mettre en lumière une réponse immunitaire à un hôte comprenant un gène codant la protéine gp140 de l'isolat primaire du VIH-1, BX08, dirigé par un promoteur d'expression de la protéine dans l'hôte, en particulier, les plasmides pCMV.gp140.BX08, pMP83, pMP84 et pMP88. On a identifié des motifs humains et murins de liaison à restriction de la classe 1 du complexe majeur d'histocompatibilité et contenus dans BX08.

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