Expression and export technology of proteins as immunofusins

Details Expression and export technology of proteins as immunofusins Expression and export technology of proteins as immunofusins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/16 (2006.01) C07K 14/475 (2006.01) C07K 14/55 (2006.01) C07K 14/705 (2006.01) C07K 14/71 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C07K 19/00 (2006.01) C12N 15/13 (2006.01) C12N 15/85 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2199830

Disclosed are DNAs produced by recombinant techniques for inducing the expression and subsequent secretion of a target protein. The DNAs encode, in their 5' to 3' direction, a secretion cassette, including a signal sequence and an immunoglobulin Fc region, and a target protein. The DNAs can be transfected into a host cell for the expression, production and subsequent secretion of the target protein as a fusion protein. The secreted protein can be collected from the extracellular space, and further purified as desired. The secreted fusion protein additionally can be proteolytically cleaved to release the target protein from the secretion cassette.

L'invention concerne des ADNs produits au moyen de techniques de recombinaison, afin de provoquer l'expression et la sécrétion consécutive d'une protéine cible. Les ADNs codent, dans leur sens 5' à 3', une cassette de sécrétion, comprenant une séquence de signaux et une région Fc d'immunoglobuline, ainsi qu'une protéine cible. On peut transfecter les ADNs dans une cellule hôte, afin de réaliser l'expression, la production et la sécrétion consécutive de la protéine cible sous forme de protéine de fusion. La protéine sécrétée peut être prélevée de l'espace extracellulaire et, ensuite, éventuellement purifiée. La protéine de fusion sécrétée peut, de plus, être soumise à un clivage protéolytique, de sorte que la protéine cible de la cassette de sécrétion soit libérée.

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