Expression and purification of an htlv-iii gag/env gene protein

C - Chemistry – Metallurgy – 12 – N

Patent

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530/13, 167/139,

C12N 15/48 (2006.01) C07K 14/15 (2006.01) C07K 14/16 (2006.01) C12N 1/00 (2006.01) C12N 15/66 (2006.01) C12Q 1/70 (2006.01) G01N 33/532 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/58 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1341249

The present invention provides methods and compositions for the cloning and expression of plasmids bearing genes encoding a novel protein derived from HTLV-III. This protein, which is called the gag/env protein and which contains antigenic determinants from both the core and envelope proteins of HTLV-III, can be purified to homo- geneity and used as the basis for diagnostic tests to detect the presence of antibodies against viruses associated with AIDS or the viruses themselves in human sera and other bio- logical fluids. The gag/env protein may also be formulated for use as a vaccine for protection against AIDS through prophylactic immunization.

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