Expression and secretion vector for human interferon alpha...

Details Expression and secretion vector for human interferon alpha... Expression and secretion vector for human interferon alpha...

C - Chemistry, Metallurgy

12

N

C12N 15/21 (2006.01) C07K 14/245 (2006.01) C07K 14/56 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2397527

Disclosed in this invention are: an expression vector for the secre-tive production of human interferon alpha (hIFNa) comprising a polynucleotide encoding a modified E. coli thermostable enterotoxin II signal sequence and a polynucleotide encoding hIFNa) ligated to the 3'-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, said process being capable of secreting a soluble form of active hIFN.alpha.), which does not contain an additional methionine residue at its N-terminal, into the periplasm of an E. coli cell.

Cette invention concerne: un vecteur d'expression pour la production par sécrétion de l'interféron humain alpha (hIFN.alpha.) comprenant un polynucléotide codant pour une séquence signal d'une entérotoxine thermostable de E.coli modifié et un polynucléotide codant pour (hIFN.alpha.) ligaturé sur l'extrémité 3' dudit interféron ; un micro-organisme transformé au moyen du vecteur d'expression; et un procédé d'obtention par sécrétion d'interféron humain par culture du micro-organisme, ledit procédé permettant de sécréter une forme soluble du (hIFN.alpha.) actif, qui ne renferme pas de résidu supplémentaire de méthionine sur son terminal N, dans le périplasme d'une cellule de E. coli.

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