Expression cassette

Details Expression cassette Expression cassette

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2562911

An expression cassette comprises targeting sites flanking a Cre fusion gene and a Cre coding sequence interrupted by an intron. This cassette can be used for the generation CreAoxP constructs and to reduce toxicity caused by the over-expression of Cre in target cells.

L'invention concerne une cassette d'expression comprenant des sites de ciblage flanquant un gène de fusion Cre et une séquence de codage Cre interrompue par un intron. Ladite cassette peut servir à générer des produits Cre/IoxP et réduire la toxicité due à la surexpression de Cre dans des cellules cibles.

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