Expression cassettes and methods for delivery of animal...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A23K 1/16 (2006.01) A61K 39/15 (2006.01) A61K 39/215 (2006.01) C07K 14/14 (2006.01) C07K 14/165 (2006.01) C12N 15/46 (2006.01) C12N 15/50 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2232023

The present invention provides an expression cassette for expressing vaccine antigens in a plant cell. The expression cassette includes a DNA sequence which encodes for at least one vaccine antigen which is operably linked to transcriptional and translational control regions functional in the plant cell. The vaccine antigens of the invention are useful for protection of an animal against mucosal diseases such as Transmissible Gastroenteritis Virus (TGEV) and rotavirus. The invention also provides a transgenic plant and transgenic plant seed which has been stably transformed to express a vaccine antigen which is included in an expression cassette of the invention. The transformed plant and plant cells may be from monocot or dicot plants and include, for example, corn, soybeans, sunflower, canola or alfalfa. The transgenic plants and plant seeds of the invention may be used as a feed composition for animals. Alternatively, the transgenic plant and plant seeds of the invention may provide an immunogenic composition for protecting animals against mucosal diseases after oral administration.

La présente invention concerne une cassette d'expression destinée à exprimer des antigènes vaccinaux dans une cellule végétale. Cette cassette d'expression comprend une séquence d'ADN qui code pour au moins un antigène vaccinal relié fonctionnellement à des régions témoins de transcription et de translation agissant dans la cellule végétale. Les antigènes vaccinaux selon l'invention sont utiles pour protéger un animal des maladies des muqueuses telles que celle produite par le virus transmissible de la gastro-entérite (TGEV) et le rotavirus. L'invention concerne aussi une plante transgénique et une semence de plante transgénique qui a été transformée de manière stable pour exprimer un antigène vaccinal inclus dans une cassette d'expression selon l'invention. Les plantes et cellules végétales transformées peuvent provenir de plantes monocotylédones ou dicotylédones et il peut s'agir, par exemple, de maïs, de graines de soja, de tournesol, de colza canola ou de luzerne. Les plantes transgéniques et les semences de plantes selon l'invention peuvent être utilisées comme composition d'alimentation des animaux. Elles peuvent aussi donner une composition immunogénique destinée à protéger les animaux des maladies de muqueuses après administration orale.

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