C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/64 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2075259
EXPRESSION CLONING METHOD Abstract of the Disclosure Disclosed is a process for cloning a nucleotide sequence that encodes a protein. The process includes the steps of: preparing a recombinant molecule containing the nucleotide sequence and a vector that includes an expression control element operatively attached at linkage sites to the nucleotide sequence; introducing the recombinant molecule into host cells derived from a multi-cellular organism; identifying, among the host cells into which the recombinant molecule is introduced, cells expressing the protein by a means that specifically detects the protein; attaching the cells expressing the protein to a solid surface; and recovering the recombinant molecule from the attached cells. The same process can also be used to test the specificity of an antibody in its binding to an antigen, when a DNA sequence encoding the antigen is available.
Hixson Douglas C.
Sang Junsheng
Thompson Nancy L.
Barrigar & Moss
Rhode Island Hospital
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