Expression cloning of protein targets for phospholipids

Details Expression cloning of protein targets for phospholipids Expression cloning of protein targets for phospholipids

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) A61K 38/00 (2006.01) C12N 15/10 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2329858

One aspect of the present invention relates to methods and reagents for identifying proteins or other cellular components (collectively "LBP" or "lipid binding partner"), which bind to lipids such as phospholipids, triacylglycerides, plasmalogens or sphingolipids. In preferred embodiments, the subject method is useful for identifying LBPs that bind to phospholipids such as phosphatidylserines, phosphatidylcholines (also called lecithins), phosphatidylethanolamines, phosphatidylglycerols, phosphatidylinositols, or sphingomyelins. The LBPs can be naturally occurring, such as proteins or fragments of proteins cloned or otherwise derived from cells, or can be artificial, e.g., poypeptides which are selected from random or semi-random polypeptide libraries.

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