Expression cloning processes for the discovery,...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2282811

The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.

La présente invention concerne une méthode de détection, de caractérisation et d'isolement d'acides nucléiques codant pour des protéines d'une propriété recherchée, telle qu'une localisation cellulaire particulière. De plus, cette invention permet une expression rapide desdites protéines ou glycoprotéines dans les cellules de mammifères et facilite la purification des nouvelles protéines ou glycoprotéines sécrétées. Enfin, l'invention concerne le marquage radioactif des nouvelles protéines ou glycoprotéines, l'identification rapide de sites de liaison, dont des animaux, et la production rapide de vecteurs viraux infectieux à utiliser pour le transfert de gènes.

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