Expression control sequences

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12N 15/72 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2308003

Expression control sequences are provided for the expression of proteins from a gene of interest. The gene may express viral, prokaryotic, or eukaryotic proteins. These control sequences are produced by combining phage promoter and operator/repressor systems. Expression vectors containing such expression control sequences, microorganisms transformed with such expression vectors and methods for producing viral, prokaryotic, and eukaryotic proteins using the expression control sequences, expression vectors and transformed microorganisms are also provided.

On utilise des séquences de régulation de l'expression pour l'expression de protéines provenant d'un gène à étudier. Le gène peut exprimer des protéines virales, procaryotes ou eucaryotes. Ces séquences de régulation sont produites par combinaison de promoteur phage et de systèmes opérateurs/répresseurs. Cette invention concerne également des vecteurs d'expression contenant ces séquences de régulation de l'expression, des micro-organismes transformés à l'aide de ces vecteurs d'expression ainsi que des procédés permettant de produire des protéines virales, procaryotes et eucaryotes à l'aide des séquences de régulation de l'expression, des vecteurs d'expression et des micro-organismes transformés.

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