Expression library immunization

A - Human Necessities – 61 – K

Patent

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A61K 39/00 (2006.01) C07K 14/195 (2006.01) C07K 14/295 (2006.01) C07K 14/30 (2006.01) C07K 16/00 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2216092

A general method for vaccinating against any pathogen is presented. The method utilizes expression library immunization, where an animal is inoculated with an expression library constructed from fragmented genomic DNA of the pathogen. All potential epitopes of the pathogen's proteins are encoded in its DNA, and genetic immunization is used to directly introduce one or more expression library clones to the immune system, producing an immune response to the encoded protein. Inoculation of expression libraries representing portions of the Mycoplasma pulmonis genome, was shown to protect mice from subsequent challenge by this natural pathogen. Protection against Listeria was also obtained using the method.

On présente un procédé général de vaccination, contre tout agent pathogène, qui implique de recourir à une immunisation par banque d'expressions, par laquelle on inocule à un animal une banque d'expressions construite à partir d'ADN génomique fragmenté provenant d'un agent pathogène. Tous les épitopes potentiels des protéines de cet agent sont codés dans son ADN et l'immunisation génétique permet d'introduire directement un ou plusieurs clones de banque d'expressions dans le système immunitaire, ce qui provoque une réponse immunitaire à la protéine codée. On a démontré que l'inoculation de banques d'expression représentant des parties du génome de Mycoplasma pulmonis protège des souris des provocations ultérieures de cet agent pathogène naturel. Ce procédé a aussi permis de conférer une protection contre Listeria.

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