Expression linkers

Details Expression linkers Expression linkers

C - Chemistry, Metallurgy

12

N

195/1.26

C12N 15/00 (2006.01)

Patent

CA 1201075

43 ABSTRACT OF THE DISCLOSURE A composite linker for use in the expression of a deoxynucleotide sequence coding for a desired protein comprises a plurality of deoxynucleotide sequences which are joined together in the direction of translation. The first sequence has a restriction site which is recognized and cleavable by a restriction endonuclease, the second sequence is homologous to the 3'-end of 16S ribosomal RNA, the third sequence has 3 to 11 deoxynucleotides, the fourth sequence has the initiation codon, the fifth sequence codes for an amino acid sequence which is selectively bindable to a solid phase material and the sixth sequence codes for a specific cleavage sequence comprising a sequence of one or more amino acids which is specifically recognized and cleavable by enzymatic or chemical means.

452927

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