Expression method using recombinant bombyx mori nuclear...

Details Expression method using recombinant bombyx mori nuclear... Expression method using recombinant bombyx mori nuclear...

C - Chemistry, Metallurgy

12

P

195/1.2, 195/1.2

C12P 19/34 (2006.01) C07K 14/56 (2006.01) C12N 15/866 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1284119

ABSTRACT OF THE DISCLOSURE A method of producing useful substances which comprising propagating in cultured cells or in a host a recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA is disclosed. The BmhNPV DNA is produced by recombina- tion with a double-stranded DNA containing (i) a 5'-upstream BmNPV DNA fragment originally occurring upstream from the structural gene coding for the production of polyhedral protein and also including the promoter region for the structural gene, (ii) a translational start codon and (iii) a gene coding for the production of a useful substance exogenous to the virus, with or without (iv) a 3'-downstream BmNPV DNA fragment originally occurring downstream from the structural gene coding for the production of polyhedral protein.

484915

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