Expression of alkaline phosphatase in yeast

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) C12N 1/16 (2006.01) C12N 5/10 (2006.01) C12N 9/16 (2006.01) C12N 15/66 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2352609

The invention concerns a process for the recombinant production and expression of eukaryotic alkaline phosphatase in yeast cells in which in particular a DNA coding for a eukaryotic highly active alkaline phosphatase having a specific activity above 3000 U/mg is used. The invention additionally concerns a process for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns appropriate vectors and host strains.

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