Expression of biologically active polypeptides in duckweed

Details Expression of biologically active polypeptides in duckweed Expression of biologically active polypeptides in duckweed

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/475 (2006.01) C07K 14/52 (2006.01) C07K 16/00 (2006.01)

Patent

CA 2540559

Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.

Procédés, séquences d'acide nucléique et cultures de plantes ou de boutures de pourpier transformées en vue de l'expression et de la sécrétion de polypeptides biologiquement actifs à partir de pourpier génétiquement modifié. L'expression de polypeptides de recombinaison dans le pourpier est améliorée par modification de la séquence nucléotidique de la cassette d'expression codant le polypeptide en vue d'une expression améliorée dans le pourpier. La récupération des polypeptides biologiquement actifs à partir du pourpier est améliorée grâce à la liaison du polypeptide biologiquement actif avec un peptide signal qui dirige la sécrétion du polypeptide dans le milieu de culture.

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