Expression of dna sequences derived from nocardioform...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/00 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) C12Q 1/44 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 2002890

A cloning vector contains a DNA sequence derived from a Nocardioform microorganism, and is capable of expressing the DNA sequence in a host microorganism. Recombinant cholesterol oxidase is produced by a host microbial cell transformed with a cloning vector containing cholesterol oxidase encoding DNA derived from a Nocardioform microorganism. A method for determining cholesterol oxidase in a sample of fluid from a human includes contacting the sample with the Nocardioform microorganism derived recombinant cholesterol oxidase.

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