C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.18, 195/1.
C12N 1/21 (2006.01) C07K 14/15 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01)
Patent
CA 1314504
EXPRESSION OF ENZYMATICALLY ACTIVE REVERSE TRANSCRIPTASE Abstract of The Disclosure This invention provides a plasmid which, when intro- duced into a suitable host cell and grown under appro- priate conditions, effects expression of a gene on the plasmid and production of a polypeptide having reverse transcriptase activity. The plasmid is a double- stranded DNA molecule which includes in a 5' to 3' order the following: a DNA sequence which includes an inducible promoters a DNA sequence which includes an ATG initiation codon; the central portion of the Moloney murine leukemia virus (MuLV) pol gene, said central portion including a DNA sequence which encodes the polypeptide having reverse transcriptase activity; DNA sequence which contains a gene associated with a selectable or identifiable phenotypic trait which is manifested when the vector is present in the host cell; and a DNA sequence which contains an origin of replica- tion from a bacterial plasmid capable of autonomous replication in the host cell. The invention also concerns a method for recovering purified enzymatically-active polypeptide having re- verse transcriptase activity, the polypeptide being encoded by the plasmid pB6 815.23, from a suitable host cell e.g. E. coli HB101 producing the polypeptide. finally, the invention concerns use of the polypeptide to prepare complementary DNA (cDNA).
508373
Goff Stephen P.
Roth Monica J.
Tanese Naoko
Gowling Lafleur Henderson Llp
The Trustees Of Columbia University
LandOfFree
Expression of enzymatically active reverse transcriptase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression of enzymatically active reverse transcriptase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of enzymatically active reverse transcriptase will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1248784