Expression of eukaryotic peptides in plant plastids

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/15 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/61 (2006.01) C07K 14/81 (2006.01) C12N 5/10 (2006.01) C12N 15/18 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2333148

Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

L'invention concerne des constructions et des procédés permettant d'exprimer des peptides dérivés d'organismes eukariotes dans les plastes de végétaux. Ces constructions possèdent un promoteur fonctionnel dans un plaste végétal, une séquence d'ADN codant pour un peptide dérivé d'un organisme eukariote, et une région de terminaison de transcription. D'autres éléments comprennent un marqueur sélectionnable, destiné à sélectionner des cellules végétales renfermant un plaste qui exprime le marqueur, des régions d'homologie d'ADN avec le génome dudit plaste, et éventuellement un site de liaison de ribosome relié au promoteur. Grâce aux méthodes utilisant ces constructions, des niveaux élevés de peptides eukariotes, tels que des protéines de mammifère, sont produits dans une cellule végétale par croissance de cellules végétales, dans conditions où les séquences de codage d'ADN sont exprimées de façon à produire un peptide eukaryote dans ledit plaste.

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