C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/545 (2006.01) C07K 14/71 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 9/96 (2006.01) C12N 15/11 (2006.01) C12N 15/70 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2168429
The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted- translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.
L'invention concerne l'utilisation d'un partenaire de fusion dépourvu de séquence de tête pour accroître la solubilité et l'activité de polypeptides recombinés en facilitant l'expression de protéines de fusion qui sont alors transportées hors du cytoplasme. L'invention englobe un acide nucléique codant un polypeptide de fusion comprenant un polypeptide semblable à l'interleukine-1 mature ou un polypeptide de translocation à tête supprimée, et elle englobe un polypeptide voulu, ainsi que des cellules hôtes comprenant de tels acides nucléiques et des protéines de fusion ainsi codées. L'invention a également trait à des procédés d'utilisation de tels acides nucléiques pour produire des polypeptides de fusion recombinés, des polypeptides matures voulus et leurs compositions purifiées.
Cohen Pedro Antonio
Mascarenhas Desmond
Olsen David R.
Olson Pamela S.
Zhang Yang
Borden Ladner Gervais Llp
Celtrix Pharmaceuticals Inc.
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