Expression of granular starch hydrolyzing enzymes in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/34 (2006.01) C08B 30/00 (2006.01) C12N 9/62 (2006.01) C12P 19/00 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2546659

The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

La présente invention concerne des cellules hôtes fongiques filamenteuses et, en particulier, les cellules hôtes de Trichoderma, utilisées dans la production d'enzymes hétérologues d'hydrolyse d'amidon granulaire à activité glucoamylase (GSHE). L'invention concerne également un procédé de production d'un sirop de glucose consistant à mettre en contact une pâte d'amidon granulaire obtenue d'un substrat d'amidon granulaire simultanément avec une alpha amylase et une GSHE à une température égale ou inférieure à la température de gélatinisation de l'amidon granulaire afin d'obtenir une composition de sirop de glucose.

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