C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/861 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2407714
The present invention relates to a method for producing a mammalian heparin- binding protein in a mammalian cell that can be cultured under anaerobic conditions after introducing a nucleic acid encoding as heparin-binding protein into said cell with comprising (a) introducing into said mammalian cell a nucleic acid encoding said heparin-binding protein; (b) culturing the cell of step (a) under conditions conducive to expression of said HBP; and (c) recovering said HBP from the culture medium. Furthermore, the invention relates to the recombinant mammalian cell used in the method.
L'invention concerne un procédé servant à produire une protéine mammifère de fixation d'héparine (HBP) dans une cellule mammifère qu'on peut cultiver dans des conditions anaérobies après avoir introduit un acide nucléique codant une protéine de fixation d'héparine dans ladite cellule, ce qui consiste à : (a) introduire dans ladite cellule mammifère un acide nucléique codant ladite protéine de fixation d'héparine ; (b) cultiver la cellule de l'étape (a) dans des conditions favorables au déclenchement de l'expression de ladite HBP ; (c) extraire ladite HBP du milieu de culture. Elle concerne, de plus, la cellule mammifère de recombinaison mise en application dans ce procédé.
Bjorn Soren
Flodgaard Hans Jakob
Rasmussen Poul Baad
Svendsen Ivan
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Leukotech A/s
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