C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 9/14 (2006.01) C12N 9/88 (2006.01) C12N 15/55 (2006.01) C12N 15/60 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2332700
Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.
L'invention concerne des constructions et des méthodes permettant d'exprimer des gènes tolérants aux herbicides, dans les plastes de cellules végétales. Les constructions comprennent un promoteur fonctionnel dans un plaste végétal, une séquence d'ADN capable de conférer une tolérance à au moins un composé herbicide dans une cellule végétale, lorsque ladite séquence d'ADN est transcrite dans les plastes de la cellule végétale, et une région de terminaison de transcription. On obtient une tolérance aux herbicides par transformation des plastes au moyen des constructions de l'invention, et par croissance des cellules végétales comprenant les plastes transformés dans des conditions où la séquence d'ADN est transcrite. On rend les plastes et les cellules végétales tolérants aux applications comprenant au moins un composé herbicide.
Hajdukiewicz Peter
Mcbride Kevin E.
Nehra Narender
Schaaf David J.
Stalker David M.
Calgene Llc
Mcfadden Fincham
LandOfFree
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