Expression of heterologous proteins in attenuated bacteria...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) C07K 14/33 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2182315

The invention provides a DNA construct comprising the htrA promoter sequence operably linked to a DNA sequence encoding one or more heterologous proteins, replicable expression vectors containing the constructs, and attenuated bacteria containing the constructs or vectors. The invention also provides a vaccine composition comprising an attenuated bacterium as defined above, or a fusion protein expressed from a construct as defined above, and a pharmaceutically acceptable carrier.

L'invention concerne un produit de recombinaison d'ADN comprenant le promoteur du <u>htrA</u> lié activement à une séquence d'ADN codant une ou plusieurs protéines hétérologues, des vecteurs d'expression réplicables contenant ledit produit de recombinaison, et des bactéries atténuées contenant lesdits produit ou vecteurs. L'invention porte également sur une composition de vaccin comprenant une bactérie atténuée conforme à la définition ci-dessus, ou une protéine de fusion exprimée à partir d'un produit comme défini ci-dessus, et un excipient pharmaceutiquement acceptable.

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