Expression of lipoproteins

Details Expression of lipoproteins Expression of lipoproteins

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A61K 39/02 (2006.01) A61K 39/09 (2006.01) A61K 39/106 (2006.01) C07K 1/16 (2006.01) C07K 14/195 (2006.01) C07K 14/20 (2006.01) C07K 14/205 (2006.01) C07K 14/315 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2223300

Heterologous lipidated proteins formed recombinantly are disclosed and claimed. The expression system can be E. coli. The heterologous lipidated protein has a leader sequence which does not naturally occur with the protein portion of the lipidated protein. The lipidated protein can have the Borrelia OspA leader sequence. The protein portion can be OspC, PspA, UreA, UreB, or a fragment thereof. Methods and compositions for forming and employing the proteins are also disclosed and claimed.

Cette invention concerne des protéines hétérologues lipidées obtenues par recombinaison. Le système d'expression peut être E. coli. La protéine hétérologue lipidée a une séquence de tête qui n'apparaît pas dans la nature, avec la fraction protéique de la protéine lipidée. Cette protéine lipidée peut avoir la séquence de tête Borrelia OspA. La fraction protéique peut être OspC, PspA, UreA, UreB ou un fragment de ces protéines. L'invention concerne également des procédés et des compositions de formation et d'utilisation de ces protéines.

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