Expression of mammalian proteins in pseudomonas fluorescens

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2553503

The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.

Cette invention concerne un procédé permettant d'améliorer la production d'une protéine mammifère de recombinaison au moyen de l'expression dans un Pseudomonad, en particulier dans un organisme de pseudomonas fluorescens. Ce procédé améliore la production de protéines mammifères, en particulier de protéines humaines ou dérivées d'organismes humains par rapport aux systèmes d'expression connus tels que E. coli dans des circonstances comparables. Cette invention concerne également des procédés permettant d'améliorer la production de protéines isolées mammifères, en particulier humaines.

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