Expression of multiple proteins in transgenic plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2433842

A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.

L'invention concerne un procédé pour produire des protéines multiples dans des plantes transgéniques. Une construction d'ADN à introduire dans des plantes comprend une disposition pour exprimer une protéine de fusion de deux protéines d'intérêt reliées par un domaine de liaison contenant de l'ubiquitine végétale. Lorsque la protéine de fusion est produite dans les cellules d'une plante transgénique transformée au moyen de la construction d'ADN, des enzymes natives présentes dans les cellules de la plante coupent la protéine de fusion pour libérer les deux protéines d'intérêt dans les cellules de la plante transgénique. Les protéines étant produites à partir de la même protéine de fusion, les quantités initiales des protéines dans les cellules de la plante sont approximativement les mêmes.

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