Expression of phytase in plants as a method of modifying...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/06 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2385580

The present invention provides a method of modifying plant productivity comprising expressing in a plant cell, tissue or organ one or more genes capable of facilitating a plant's ability to utilise soil phosphorus, and, in particular, a phytase gene, wherein said phytase gene expresses a phytase polypeptide in secretable form. More particularly, the present invention provides a method of increasing plant productivity comprising expressing in the root of a plant an isolated nucleic acid molecule encoding a phytase polypeptide for a time and under conditions sufficient for said phytase to be secreted from the root. In a preferred embodiment of the invention the chemistry of the soil around the root is modified by the application of an organic acid. The invention also provides novel phytase-encoding genes; genetic constructs which are useful for performing the inventive method; and transgenic plants produced therewith having improved productivity compared to their otherwise isogenic counterparts.

L'invention porte sur un procédé modifiant le rendement de plantes consistant à faire exprimer dans une cellule, un tissu ou un organe de plante un ou plusieurs gènes capables d'en faciliter la capacité à utiliser le phosphore du sol, et en particulier un gène de phytase exprimant un polypeptide de phytase sous forme sécrétable. L'invention porte plus particulièrement sur un procédé d'accroissement de la productivité de plantes consistant à faire exprimer dans la racine de la plante une molécule isolée d'acide nucléique codant pour un polypeptide de phytase pendant un temps et sous des conditions suffisants pour que ladite phytase soit sécrétée par la plante. Dans l'exécution préférée la chimie du sol autour de la racine est modifiée par application d'un acide organique. L'invention porte également sur de nouveaux gènes codant pour ladite phytase, sur des produits d'assemblage géniques servant à mettre en oeuvre le procédé de l'invention, et sur des plantes transgéniques produites par son intermédiaire présentant des rendements améliorés par rapport à leurs pendants isogéniques.

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