Expression of polypeptides from the nuclear genome of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/12 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2677475

A method of producing at least one polypeptide from the nuclear genome of Ostreo-coccus sp., the method including introducing at least one recombinant nucleic acid molecule into the nuclear genome of Ostreococcus sp., wherein the recombinant nucleic acid molecule included a first polynucleotide operatively linked to a second polynucleotide, wherein the second polynucleotide encodes at least one polypeptide and wherein the first polynucleotide comprises a promoter sequence allowing expression of the at least one polypeptide in Ostreococcus sp.

La présente invention concerne un procédé de production d'au moins un polypeptide provenant du génome de l'ostréococcus sp., le procédé comportant l'étape consistant à introduire au moins une molécule d'acide nucléique recombinée. La molécule d'acide nucléique recombinée comporte un premier polynucléotide lié de façon opérationnelle à un second polynucléotide. Le second polynucléotide encode au moins un polypeptide et le premier polynucléotide comprend une séquence de promoteur permettant l'expression du ou des polypeptides dans l'ostréococcus sp.

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