Expression of processed recombinant lactoferrin and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 14/79 (2006.01) C12N 1/15 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/65 (2006.01) C12N 15/80 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2204246

The subject invention provides for the production of lactoferrins and lactoferrin polypeptide fragments using the host cells Aspergillus in combination with novel plasmid constructs. More specifically, the subject invention provides novel vector constructs capable of producing lactoferrins and lactoferrin polypeptide fragments in Aspergillus host cells. More particularly, the subject invention provides for novel plasmid constructs suitable for use with Aspergillus and especially Aspergillus awamori, niger and oryzae host cells, which enables them to produce large amounts of recombinant lactoferrins and lactoferrin polypeptide fragments.

L'invention concerne la production de lactoferrines et de fragments polypeptidiques de lactoferrines à l'aide de cellules hôtes d'Aspergillus combinées avec de nouveaux produits d'assemblage plasmidiques. Plus précisément, elle concerne de nouveaux vecteurs, résultant d'un assemblage, qui peuvent produire des lactoferrines et des fragments polypeptidiques de lactoferrines dans des cellules hôtes d'Aspergillus. Elle concerne notamment de nouveaux produits d'assemblage plasmidiques convenant pour une utilisation avec des cellules hôtes d'Aspergillus et notamment d'Aspergillus awamori, niger et oryzae, ce qui leur permet de produire de grandes quantités de lactoferrines et de fragments polypeptidiques de lactoferrines recombinés.

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