Expression of proteins on bacterial surface

Details Expression of proteins on bacterial surface Expression of proteins on bacterial surface

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) A61K 39/39 (2006.01) C07K 14/245 (2006.01) C07K 16/00 (2006.01) C12N 9/16 (2006.01) C12N 9/86 (2006.01) C12N 11/16 (2006.01) C12N 15/10 (2006.01) C12N 15/13 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2123676

2123676 9310214 PCTABS00022 The invention relates to a method for producing stable, surface-expressed polypeptides from recombinant gram-negative bacterial cell hosts. A tripartite chimeric gene and its related recombinant vector include separate DNA sequences for directing or targeting and translocating a desired gene product from a cell periplasm to the external cell surface. A wide range of polypeptides may be efficiently surface expressed, including .beta.-lactamase and alkaline phosphatase. Full enzyme activity is maintained and the proteins remain anchored to the bacterial outer membrane surface.

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