Expression of recombinant fusion proteins in attenuated...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/21 (2006.01) A61K 39/08 (2006.01) A61K 39/112 (2006.01) A61K 39/116 (2006.01) A61K 39/295 (2006.01) C07K 14/005 (2006.01) C07K 14/025 (2006.01) C07K 14/035 (2006.01) C07K 14/09 (2006.01) C07K 14/195 (2006.01) C07K 14/255 (2006.01) C07K 14/33 (2006.01) C07K 19/00 (2006.01) C12N 9/52 (2006.01) C12N 15/31 (2006.01) C12N 15/33 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2141427

The invention provides a DNA molecule comprising a promoter sequence op-erably linked to a DNA se-quence encoding first and second proteins linked by a hinge region wherein. in that the promoter sequence can be one having activity which is induced in response to a change in the surrounding environment and the first protein can be Tetanus toxin C fragment or one or more epitopes thereof. The inven-tion also provides intermedi-ate molecules having a prom-oter operably linked to a DNA sequence encoding a first antigenic sequence and a hinge region, and at or adja-cent the 3'-end thereof one or more restriction sites for the introduction of a second anti-genic sequence. In addition, the invention provides repli-cable expression vectors containing the DNA fusion proteins expressed therefrom, bacterial transformed with the vectors and the use of the bacteria, in vaccines.

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