Expression of recombinant polypeptides with improved...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/58 (2006.01) C07K 19/00 (2006.01) C12N 15/16 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2089094

An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fu- sion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell pro- teins, and separation of the carrier from the peptide after cleavage.

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