Expression of soluble antibody fragment by truncation of ch1...

Details Expression of soluble antibody fragment by truncation of ch1... Expression of soluble antibody fragment by truncation of ch1...

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C07K 16/00 (2006.01) C12N 15/13 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2689556

Improved expression of active antibody fragments (Fabs) is achieved by truncating a heavy chain constant region. Truncation of the CHI domain of a Fab fragment can increase yield of soluble active antibody fragment in Pseudomonas fluorescens. Another embodiment of the invention includes secretion of the light chain and a fragment of the heavy chain with various C-termini (e.g., VH- CHI truncated to different lengths). The truncated CHI region can be used as a scaffold to create other Fabs. Also included is truncation of the kappa light chain and/or lambda light chain domains of a Fab fragment. The invention also includes expression of Fab fragments fused to other peptides or molecules (e.g., toxins, proteins, peptides, enzymes, etc.).

L'invention concerne l'expression améliorée de fragments d'anticorps actifs (Fab) par troncature d'une région constante à chaîne lourde. La troncature du domaine CHI d'un fragment Fab peut augmenter la production de fragment d'anticorps actif soluble dans Pseudomonas fluorescens. Un autre mode de réalisation de l'invention comprend la sécrétion de la chaîne légère et d'un fragment de la chaîne lourde avec diverses terminaisons C (par exemple, VH- CHI tronqué sur différentes longueurs). La région CHI tronquée peut être utilisée comme échafaudage pour créer d'autres Fab. La troncature des domaines à chaîne légère kappa et/ou à chaîne légère lambda d'un fragment Fab est également incluse. L'invention comprend également l'expression de fragments Fab fusionnés avec d'autres peptides ou molécules (par exemple, toxines, protéines, peptides, enzymes, etc.).

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