Expression of the alpha-toxin gene (hla) of staphylococcus...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/74 (2006.01) C07K 14/31 (2006.01) C12N 1/21 (2006.01) C12Q 1/02 (2006.01) C12Q 1/54 (2006.01)

Patent

CA 2208724

The present invention relates to a Staphylococcus aureus strain containing an reporter gene operatively linked to the promoter of the hla-gene, wherein the reporter gene is the lacZ gene; a bacterial strain, obtainable by transforming staphylococcus aureus wood 46 with hla-lacZ fusion gene using the recombinant plasmid pkO10 carrying said fusion gene, the bacterial strain Staphyloccocus aureus Wood 46-3; the use of a bacterial strain according to any of the proceeding claims to measure the hla promoter activity under different growth conditions, wherein the different growth conditions are selected from the group consisting of length of growth time and phase, temperature, osmolarity, glucose concentration,solid/liquid media, oxygen concentration, and concentration of low, subinhibitory concentrations of antibiotics; the vector pkO10, and a process for the production of Staphylococcus aureus 46-3.

La présente invention concerne une souche de Staphylococcus aureus contenant un gène reporter lié fonctionnellement au promoteur du gène hla et où le gène reporter est le gène lacZ; une souche bactérienne, que l'on peut obtenir par transformation de Staphylococcus aureus Wood 46 par le gène de fusion hla-lacZ à l'aide du plasmide recombinant pkO10 portant ledit gène de fusion, la souche bactérienne Staphylococcus aureus Wood 46-3; l'utilisation d'une souche bactérienne conformément à n'importe quelle des revendications précédentes pour mesurer l'activité du promoteur hla dans différentes conditions de croissance, où les conditions de croissance sont choisies parmi les conditions suivantes : durée de la période et de la phase de croissance, température, osmolarité, concentration de glucose, milieux liquides-solides, concentration d'oxygène; concentrations faibles, infra-inhibitrices d'antibiotiques; le vecteur pkO10 et un procédé pour la production de Staphylococcus aureus 46-3.

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