Expression plasmid, a fusion protein, a transfected...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/85 (2006.01) A61K 38/36 (2006.01) A61K 38/38 (2006.01) A61K 38/48 (2006.01) A61K 39/12 (2006.01) C07K 14/005 (2006.01) C07K 14/745 (2006.01) C07K 14/755 (2006.01) C07K 14/765 (2006.01) C12N 9/64 (2006.01) C12N 9/74 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) C12N 15/65 (2006.01) C12N 15/67 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2162770

The invention describes an expression plasmid containing a dicistronic transcription/translation unit, which unit comprises a sequence for a foreign protein and a sequence for a fusion protein, the fusion protein containing at least one selection marker and at least one amplification marker. Further described is a method of producing foreign proteins by using the plasmids according to the invention, as well as cell lines transformed with the plasmid according to the invention.

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